Visualization of retrovirus budding with correlated light and electron microscopy.

Comparing Invasive and Non-Invasive of Isolated Shigella flexneri by Electron Microscopy of Cell Culture, SDS-PAGE and Congo Red Method

The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. Methods: S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children’s hospitals. Phenotype analysis for screening v...

1 Correlated Light - Serial Scanning Electron Microscopy ( CoLSSEM ) for ultrastructural visualization of single neurons in vivo

Search for retrovirus-like particles in human breast cancer cells in culture.

We have recently established four new human breast cancer cell lines that were characterized as being of human mammary origin. We examined these cell lines for particles morphologically resembling retroviruses by electron microscopy, for extracellular and intracellular particles containing high-molecular-weight RNA and RNA-directed DNA polymerase by biochemical assays, and for mouse mammary tum...

Visualization of Assembly Intermediates and Budding Vacuoles of Singapore Grouper Iridovirus in Grouper Embryonic Cells

Iridovirid infection is associated with the catastrophic loss in aquaculture industry and the population decline of wild amphibians and reptiles, but none of the iridovirid life cycles have been well explored. Here, we report the detailed visualization of the life cycle of Singapore grouper iridovirus (SGIV) in grouper cells by cryo-electron microscopy (cryoEM) and tomography (ET). EM imaging r...

Imaging endocytic clathrin structures in living cells.

Our understanding of the clathrin-dependent endocytic pathway owes much to new visualization techniques. Budding coated pits and clathrin-coated structures are transient molecular machines with distinctive morphological characteristics, and fluorescently labeled versions of a variety of marker proteins have given us a tantalizing glimpse of the dynamics of the system in living cells. Recent liv...